Resurrecting a miniature Cas9 ancestor for genome and epigenome editing

Nature Biotechnology, Published online: 03 June 2025; doi:10.1038/s41587-025-02707-8The ancestral protein of the CRISPR–Cas9 system — less than half the size of its famous descendant — is engineered into an effective DNA-targeting tool that can be delivered by AAV as an epigenome editor for in vivo gene silencing…
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